■ 基本信息
啟動(dòng)子: | GAL1 |
復(fù)制子: | 2μFI |
終止子: | CYC1 |
質(zhì)粒分類: | 酵母系列，釀酒酵母表達(dá)載體 |
質(zhì)粒大小: | 5870bp |
原核抗性: | Amp |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 酵母細(xì)胞 |
誘導(dǎo)方式: | 半乳糖誘導(dǎo) |
5'測序引物:
| GAL1-F:ATTTTCGGTTTGTATTACTTC
|
3'測序引物: | GALI-R:GTTCTTAATACTAACATAACT |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | TRP1
|
熒光標(biāo)記: |
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■ 質(zhì)粒簡介
pYES3/CT and pYES6/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Vectors feature a C-terminal V5 epitope for detection with an Anti-V5 Antibody. pYES3/CT carries the TRP1 selection marker for selection in yeast. pYES6/CT carries the bsd resistance gene for selection with the antibiotic Blasticidin, a potent selection agent that can be used at very low concentrations and in any strain regardless of auxotrophic marker.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK952pYES3CT釀酒酵母質(zhì)粒.txt
