■ 基本信息
啟動子: | ADH1 |
復(fù)制子: | 2μ |
質(zhì)粒分類: | 酵母系列，酵母雙雜交載體 |
質(zhì)粒大小: | 10.kb |
原核抗性: | Amp |
真核抗性: | LEU2 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 酵母細胞 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | 根據(jù)序列設(shè)計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 雜交 |
片段類型: |
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: | LEU2
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡介
pGADT7-T encodes a fusion of the SV40 large T-antigen (a.a. 86–708) and the GAL4 AD (a.a. 768–881). The SV40 large T DNA (GenBank Locus SV4CG) was derived from a plasmid referenced in Li & Fields (1993) and was cloned into pGADT7 using the EcoR I and Xho I sites. pGADT7-T has not been sequenced.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列可參考（具體以測序為準(zhǔn)）下載：
ZK976 pGADT7-T酵母雙雜交質(zhì)粒.txt
