■ 基本信息
啟動(dòng)子: | ADH1 |
復(fù)制子: | 2μ |
質(zhì)粒分類(lèi): | 酵母系列，酵母雙雜交載體 |
質(zhì)粒大小: | 8.3kb |
原核抗性: | Kan |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 酵母細(xì)胞 |
5'測(cè)序引物: | T7:TAATACGACTCACTATAGGG |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 雜交 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | TRP1
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
Yeast two-hybrid systems provide a sensitive method for detecting relatively weak and transient protein interactions. Such interactions may not be biochemically detectable, but may be critical for proper functioning of complex biological systems. pGBKT7-53 and pGADT7-T encode fusions between the GAL4 DNA-BD and AD and murine p53 and SV40 large T-antigen, respectively. p53 and large T-antigen interact in a yeast two-hybrid assay.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列可參考（具體以測(cè)序?yàn)闇?zhǔn)）下載：
ZK981 pGBKT7-53酵母雙雜交質(zhì)粒.txt
